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Mouse Monoclonal anti-LAP2 (6E10)
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CatalogCode:NB120-11823
ProductName:LAP2 Antibody
Product Description:Mouse Monoclonal anti-LAP2 (6E10)
Clone:6E10
Clonality:Monoclonal
Immunogen:Synthetic peptide: GEQRKDVYVQLYLQHLTARNRP, corresponding to amino acids 29-50 of Human LAP2, common to all isoforms.
Epitope:The epitope recognized by the antibody resides within the N-terminus (amino acids 29-50), common to all human and mouse LAP2/TMPOs
CrossReactivity:Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (90% identity with immunogen) and Cow (88% identity with immunogen) due to sequence homology.Not yet tested in other species.
Packaging:0.1 ml purified Mouse IgG.
Uses:IHC-P: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 4 ug/ml. Detects bands of approximately 76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Localization:Nuclear and Secreted
Control:Whole extract of cultured human chronic myelogenous leukemia K562 cells.
Storage:Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Isotype:IgG1
Host_Name:Mouse
Buffer:Preservative: 15mM Sodium Azide Constituents: 0.01M PBS. pH 7.4
ListPrice:285
AppSummary:IHC-P, WB
SpeciesSummary:Hu
ProteinTarget:LAP2
PackageSize:0.1 ml
GeneralRef:General / background references:Nili E et al. Nuclear membrane protein LAP2beta mediates transcriptional repression alone and together with its binding partner GCL (germ-cell-less). J Cell Sci 114:3297-307 (2001). Alsheimer M et al. Architecture of the nuclear periphery of rat pachytene spermatocytes: distribution of nuclear envelope proteins in relation to synaptonemal complex attachment sites. Mol Biol Cell 10:1235-45 (1999). Goldberg M et al. The nuclear lamina: molecular organization and interaction with chromatin. Crit Rev Eukaryot Gene Expr 9:285-93 (1999).
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