| CatalogCode: | NB120-11325 |
| ProductName: | Tubulin Antibody |
| Product Description: | Mouse Monoclonal anti-Tubulin (TUB-1A2) |
| Clone: | TUB-1A2 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide containing the carboxy terminal amino acids of alpha tubulin. |
| Specificity: | The antibody reacts against tubulin's C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections. |
| CrossReactivity: | Cross-reacts with tyrosine tubulin from the following sources: bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumor (AtT-20), yeast, and Xenopus.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/800. This concentration was determined for cultured chicken fibroblasts.WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Centrosomal. |
| Background: | The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG3 |
| Host_Name: | Mouse |
| Buffer: | Preservative: 0.1% Sodium Azide Constituents: Ascites fluid. |
| ListPrice: | 285 |
| AppSummary: | IF, WB |
| SpeciesSummary: | All |
| ALTnames: | anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody, anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody |
| ProteinTarget: | Tubulin Tyrosine |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Kreis TE Microtubules containing detyrosinated tubulin are less dynamic. EMBO J 6:2597-606 (1987). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |