| CatalogCode: | NB120-11324 |
| ProductName: | Tubulin - Polyglutamylated Antibody |
| Product Description: | Mouse Monoclonal anti-Tubulin - Polyglutamylated (B3) |
| Clone: | B3 |
| Clonality: | Monoclonal |
| Immunogen: | Purified sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Epitope: | The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubulins (the glutamylated motif at amino acid 445-457 of alpha tubulin). |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Chicken, Macaque Monkey, Rabbit and Sea Urchin. Not yet tested in other species. |
| Packaging: | 0.2 ml Immunogen affinity purified Mouse ascites. |
| Uses: | ICC: Can be used with 4% paraformaldehyde-1% Triton X-100 and methanol-acetone.WB: The concentration is recommended using a cytosolic fraction of rat brain extract. Detects a band of approximately 50 kDa, possibly a doublet of alpha and beta tubulins. |
| Localization: | Centrosomal. |
| Control: | The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubul |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgM |
| Host_Name: | Mouse |
| Buffer: | 1% BSA, 0.01M PBS. pH 7.4 |
| ListPrice: | 325 |
| AppSummary: | ELISA, WB, ICC |
| SpeciesSummary: | Bv, Ch, Hu, Mu, Mk, Rt, Rb |
| ALTnames: | anti-Alpha tubulin ubiquitous antibody; anti-H2 ALPHA antibody; anti-TUBA1 antibody; anti-Tubulin alpha 1 chain antibody; anti-Tubulin alpha antibody; anti-Tubulin alpha ubiquitous chain antibody; anti-Tubulin K alpha 1 antibody |
| ProteinTarget: | Tubulin Polyglutamylated |
| PackageSize: | 0.2 ml |
| GeneralRef: | General / background references:Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). Bobinnec Y et al. Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells. J Cell Biol 143:1575-89 (1998). Gagnon C et al. The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility. J Cell Sci 109 ( Pt 6):1545-53 (1996). Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
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