| CatalogCode: | NB120-11314 |
| ProductName: | beta III Tubulin Antibody |
| Product Description: | Mouse Monoclonal anti-beta III Tubulin (SDL.3D10) |
| Clone: | SDL.3D10 |
| Clonality: | Monoclonal |
| Immunogen: | Chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype III conjugated to BSA. |
| Epitope: | Ab11314 specifically recognizes an epitope located on human beta-tubulin (isotype III). |
| Specificity: | Ab11314 may be used for the localization of isotype III of beta-tubulin using various immunochemical assays. |
| CrossReactivity: | Cross-reacts with Human, Rat and Cow.Does not cross-react with Chicken.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Tubulin is the major constituent of microtubules. |
| Control: | Rat brain extract. |
| Background: | Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: Raw Ascites |
| ListPrice: | 285 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu, Rt, Bv, Ch(-) |
| ALTnames: | anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-TUBB4 antibody, anti-Tubulin beta 3 antibody, anti-Tubulin beta 4 antibody |
| ProteinTarget: | beta III Tubulin Neuronal Marker |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). Banerjee A et al. A monoclonal antibody against the type II isotype of beta-tubulin. Preparation of isotypically altered tubulin. J Biol Chem 263:3029-34 (1988). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |