| CatalogCode: | NB120-11313 |
| ProductName: | beta I + II Tubulin Antibody |
| Product Description: | Mouse Monoclonal anti-beta I + II Tubulin (JDR.3B8) |
| Clone: | JDR.3B8 |
| Clonality: | Monoclonal |
| Immunogen: | A chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype II coupled to BSA. |
| Epitope: | Ab11313 specifically recognizes an epitope located on the isotypes I and II of human beta-tubulin. |
| Specificity: | Using the immunoblotting technique, the antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line. Using the immunofluorescence technique, the antibody localizes the beta tubulin isotypes I and II in human and chicken fibroblasts. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Chicken and Pig.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | IF: Use at an assay dependent dilution. The antibody localizes the beta-tubulin isotypes I and II in human and chicken fibroblasts.WB: Use at a dilution of 1/1000. The antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line.Predicted molecular weight: 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Tubulin is the major constituent of microtubules. |
| Control: | Chicken fibroblasts extract, chicken brain tubulin preparation. |
| Background: | Tubulin is the major building block of microtubules. This intracellular, cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar but not identical sets of alpha and beta tubulin polypeptides. Within either family, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxy-terminal 15 residues. For beta-tubulin, five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species with the exception of the hematopoietic beta tubulin which is highly divergent in sequence and which is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | Preservative: 0.1% Sodium Azide Constituents: Raw Ascites |
| ListPrice: | 285 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Hu, Mu, Rt, Bv, Po |
| ProteinTarget: | beta I + II Tubulin |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). Banerjee A et al. A monoclonal antibody against the type II isotype of beta-tubulin. Preparation of isotypically altered tubulin. J Biol Chem 263:3029-34 (1988). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
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