| CatalogCode: | NB120-11309 |
| ProductName: | beta Tubulin Antibody |
| Product Description: | Mouse Monoclonal anti-beta Tubulin (TUB 2.1), conjugated to Cy3 |
| Clone: | TUB 2.1 |
| Clonality: | Monoclonal |
| Immunogen: | Purified rat brain tubulin. |
| Epitope: | Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446). |
| Specificity: | It reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain beta-tubulin isoform-related specificity |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Hamster, Frog, Moth, Wheat and Rabbit.Not yet tested in other species. |
| Packaging: | 0.2 ml purified Mouse IgG. |
| Uses: | ICC: Use at an assay dependent dilution. IF: Use at a dilution of 1/100, this dilution was determined by direct immunofluorescent labeling of cultured chicken fibroblasts or BHK cells. The conjugate may be used in double labeling experiments with fluorescein tagged antibodies.IHC: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Major constituent of microtubules. |
| Control: | Cultured chicken fibroblasts or BHK cells. |
| Background: | Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | ICC, IF, IHC |
| ALTnames: | anti-M40 antibody, anti-Tubulin beta polypeptide antibody, anti-Tubulin beta-2 chain antibody |
| ProteinTarget: | beta Tubulin |
| PackageSize: | 0.2 ml |
| Conjugate: | Cy3 |
| GeneralRef: | General / background references:Southwick PL et al. Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters. Cytometry 11:418-30 (1990). Matthes T et al. Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule. J Immunol 141:3135-41 (1988). Gozes I & Barnstable CJ Monoclonal antibodies that recognize discrete forms of tubulin. Proc Natl Acad Sci U S A 79:2579-83 (1982). |
| NotesMain: | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
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