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| Catalog Code: | NB120-11268 |
| Product Name: | MAP2 Antibody |
| Product Description: | Mouse Monoclonal anti-MAP2 (AP-20) |
| Clone: | AP-20 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Bovine protein. |
| Specificity: | The antibody localizes the high molecular weight forms of MAP2 namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No cross-reactivity is observed with MAP1, MAP5, tubulin, or tau. |
| Cross Reactivity: | Cross-reacts with Mouse, Rat, Xenopus laevis, Cow, Quail and Salamander. |
| Packaging: | 0.05 ml purified Mouse ascites. |
| Uses: | ICC: Use at an assay dependent dilution. Use either a fluorescent or peroxidase label. Western Blot: Use at an assay dependent dilution. Detects a band of approximately 280 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Application Summary: | Immunocytochemistry, Western Blot, |
| Species Summary: | Mu, Rt, Xp, Bv, Av, Am |
| Alternate Names: | anti-Dendrite specific MAP antibody, anti-DKFZp686I2148 antibody, anti-MAP 2 antibody, anti-MAP2 antibody, anti-MAP2A antibody, anti-MAP2B antibody, anti-MAP2C antibody, anti-Microtubule associated protein 2 antibody, anti-Mtap 2 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 15mM Sodium Azide |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| General References: | General / background references:Binder LI et al. Differential localization of MAP-2 and tau in mammalian neurons in situ. Ann N Y Acad Sci 466:145-66 (1986). |
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