| CatalogCode: | | NB120-11262 |
| ProductName: | | Kinesin Antibody |
| Product Description: | | Mouse Monoclonal anti-Kinesin (IBII) |
| Clone: | | IBII |
| Clonality: | | Monoclonal |
| Immunogen: | | Bovine brain kinesin. |
| Specificity: | | The antibody recognizes the kinesin heavy chain. |
| CrossReactivity: | | Cross-reacts with Rat, Cow and Sea Urchin. Not yet tested in other species. |
| Packaging: | | 0.1 ml purified Mouse ascites. |
| Uses: | | ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. Ab11262 recognizes kinesin in denatured and reduced tissue or cell preparations of the following species: rat (brain), bovine (microtubules or kinesin enriched brain), sea urchin eggs (microtubules). In kinesin-enriched rat brain preparation, 2 bands are detected at approximately 130-140 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Microtubules |
| Control: | | Rat brain preparation. |
| Background: | | Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function. |
| Storage: | | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgM |
| Host_Name: | | Mouse |
| Buffer: | | Preservative: 0.1% Sodium Azide Constituents: Raw Ascites |
| ListPrice: | | 285 |
| AppSummary: | | ELISA, WB |
| ALTnames: | | anti-KIF5B antibody, anti-Kinesin 1 (110-120kD) antibody, anti-Kinesin family member 5B antibody, anti-Kinesin heavy chain ubiquitous antibody, anti-KINH antibody, anti-KNS1 antibody, anti-Ubiquitous kinesin heavy chain antibody, anti-UKHC antibody |
| ProteinTarget: | | Kinesin |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | General / background references:Brady ST et al. A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm. Proc Natl Acad Sci U S A 87:1061-5 (1990). Yang JT et al. Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science 249:42-7 (1990). Ingold AL et al. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J Cell Biol 107:2657-67 (1988). Brady ST A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317:73-5 (1985). |
| NotesMain: | | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| more information: | | company product webpage |