| CatalogCode: | NB120-11193 |
| ProductName: | Vinculin Antibody |
| Product Description: | Mouse Monoclonal anti-Vinculin (VIN-11-5) |
| Clone: | VIN-11-5 |
| Clonality: | Monoclonal |
| Immunogen: | smooth muscle vinculin from chicken gizzard. |
| Specificity: | The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Cow and Chicken.Not yet tested in other species. |
| Packaging: | 0.2 ml purified Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/50. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide |
| ListPrice: | 285 |
| AppSummary: | IF |
| ALTnames: | anti-Metavinculin antibody, anti-MVCL antibody, anti-VCL antibody, anti-VINC antibody |
| ProteinTarget: | Vinculin |
| PackageSize: | 0.2 ml |
| GeneralRef: | General / background references:Gimona M et al. Smooth muscle specific expression of calponin. FEBS Lett 274:159-62 (1990). Volberg T et al. Changes in membrane-microfilament interaction in intercellular adherens junctions upon removal of extracellular Ca2+ ions. J Cell Biol 102:1832-42 (1986). |
| NotesMain: | Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
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