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| Catalog Code: | NB120-11099 |
| Product Name: | Nebulin Antibody |
| Product Description: | Mouse Monoclonal anti-Nebulin (NB2) |
| Clone: | NB2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Full length native protein (purified) (Chicken). |
| Specificity: | Does not cross react with titin. Monoclonal Anti-Nebulin reacts only with skeletal muscle, not with smooth muscle or different non-muscle tissues and cultured cells.The following is a summary of the antibody reactivity on various muscles in immunofluorescence using frozen section (a), isolated myofibrils (b), or immunoblotting (c):Fish skeletal muscle (a) (Torpedo spec.) +Toad skeletal muscle (a) (Bufo marinus) +Salamander skeletal muscle (c) (Ambystoma tigrina) +Salamander heart muscle (a) -Alligator skeletal muscle (a)(Alligator mississippiensis) +Pigeon skeletal muscle (b) (c) +Chicken skeletal muscle (b) (c) +Chicken heart muscle (c) -Chicken gizzard smooth muscle (c) -Mouse skeletal muscle (a) +Rat skeletal muscle (b) (c) +Rat heart muscle (a) -Rat rhabdomyosarcoma (a) +Rabbit skeletal muscle (b) +Bovine heart muscle (a) -Pig skeletal muscle (c) +Human skeletal muscle (a) + |
| Cross Reactivity: | Cross-reacts with a wide range of species, including chicken, mouse, rat, rabbit, cow, pig, human, salamander, pigeon, alligator, toad and fish. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/400. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 um from the z-line in electron micrographs. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Nebulin is a high molecular weight protein (500-800 kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Preservative: 0.1% Sodium Azide |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Ch, Mu, Rt, Rb, Bv, Po, Am, Av, Al, Fi |
| Alternate Names: | anti-DKFZp686C1456 antibody, anti-FLJ39568 antibody, anti-FLJ39584 antibody, anti-NEB 177D antibody, anti-NEB antibody, anti-NEB177D antibody, anti-NEM2 antibody |
| Package Size: | 0.1 ml |
| Notes Main: | If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
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