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| Catalog Code: | NB120-11012 |
| Product Name: | Band 3 Antibody |
| Product Description: | Mouse Monoclonal anti-Band 3 (BIII 136) |
| Clone: | BIII 136 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 6.0 mg/ml |
| Immunogen: | Full length native protein (purified). |
| Epitope: | The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kD from the N-terminal end. |
| Specificity: | This antibody was found to be specific for the cytoplasmic amino-terminal protein of band 3. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD.Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.This antibody does not react with human fibroblast extract (nonerythroid). |
| Cross Reactivity: | Cross-reacts with Human. Does not cross-react with Pig, Mouse, Cow, Dog, Guinea pig or Horse. Not yet tested in other species. |
| Packaging: | 0.1 ml Mouse ascites. |
| Uses: | Immunofluorescent staining of fixed human erythrocytes. WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane; multi-pass membrane protein |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Buffer: | Constituents: Raw Ascites |
| Application Summary: | ELISA, immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Bv(-), Ca(-), Hu, Mu(-), Po(-), Gp(-), Eq(-), |
| Alternate Names: | Anti-AE 1 antibody; Anti-AE1 antibody; Anti-Anion exchange protein 1 antibody; Anti-Anion exchanger 1 antibody; Anti-Band 3 anion exchange protein antibody; Anti-Band 3 anion transport protein antibody; Anti-BND 3 antibody; Anti-BND3 antibody; Anti-CD 233 antibody; Anti-CD233 antibody; Anti-CD233 antigen antibody; Anti-DI antibody; Anti-EMPB 3 antibody; Anti-EMPB3 antibody; Anti-EPB 3 antibody; Anti-EPB3 antibody; Anti-Erythrocyte membrane protein band 3 antibody; Anti-Erythrocyte membrane protein band 3 Diego blood group antibody; Anti-Erythroid anion exchange protein antibody; Anti-FR antibody; Anti-Froese blood group antibody; Anti-MGC116750 antibody; Anti-MGC116753 antibody; Anti-MGC126619 antibody; Anti-MGC126623 antibody; Anti-RTA1A antibody; Anti-SLC4A1 antibody; Anti-Solute carrier family 4 anion exchanger member 1 antibody; Anti-Solute carrier family 4 member 1 antibody; Anti-SW antibody; Anti-Swann blood group antibody; Anti-Waldner blood group antibody; Anti-WD 1 antibody Anti-WD antibody; Anti-WD1 antibody; Anti-WR antibody; Anti-Wright blood group antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% Sodium Azide |
| Notes Main: | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| General References: | General / background references:Czerwinki M et al. Degradation of the human erythrocyte membrane band 3 studied with monoclonal antibody directed against an epitope on the cytoplasmic fragment of band 3. Eur J Biochem 174:647-54 (1988). |
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