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| Catalog Code: | NB120-11003 |
| Product Name: | Actin Antibody |
| Product Description: | Mouse Monoclonal anti-Actin (AC-40) |
| Clone: | AC-40 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 3.2 mg/ml |
| Immunogen: | Synthetic peptide: SGPSIVHALCP (human) attached to Multiple Antigen Peptide (MAP) backbone. |
| Epitope: | Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms. |
| Specificity: | The antibody does not react with the epitope on the N-terminal end of actin. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Carp, Chicken, Dog, Frog, Goat, Guinea pig, Hamster, Pig, Rabbit, Sheep, Snail and Viper. Not yet tested in other species. |
| Packaging: | 0.1 ml Ascites Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/2000 determined by indirect immunofluorescent staining of cultured human or chicken fibroblasts.IHC-P: Use at an assay dependent dilution. Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel?s or Bouin?s solution may also be used as fixatives.IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/500 using cultured human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis.1-3 Four of them represent the differentiation markers of muscle tissues and two are found in nearly all cells. There are three a-actins (a-skeletal, a-cardiac and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues.4 The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin.5 The actin in cells of various species and tissues are very similar in their immunological and physical properties. As a consequence, it is difficult to produce antisera to this protein. The availability of monoclonal antibody to actin provides a specific and useful tool in studying actin structure and function and in probing binding sites of actin-binding proteins. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Application Summary: | ELISA , immunohistochemistry , immunofluorescence 1/200, Western Blot 1/500, Immunohistochemistry-Paraffin , Dot |
| Species Summary: | Bv, Ca, Ch, Ha, Hu, Mu, Po, Rt, Rb, Gp, Am, Gt, Sh, |
| Alternate Names: | anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody, anti-DFNA20 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 15mM Sodium Azide |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| General References: | 1. Herman, I., Curr. Opin. Cell Biol., 5, 48 (1993). 2. Vanderkerckhove, J., and Weber, K., Eur. J. Biochem., 90, 451 (1978). 3. Drew, J., et al., Amer. J. Physiol., 260, C1332 (1991). 4. Lessard, J., Cell Motil. Cytoskel., 10, 349 (1988). 5. Sutoh, K., and Mabuchi, I., Biochemistry, 25, 6186 (1986). |
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