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| Catalog Code: | NB120-10966 |
| Product Name: | Placental Growth Factor Antibody |
| Product Description: | Mouse Monoclonal anti-Placental Growth Factor (37203.111) |
| Clone: | 37203.111 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Recombinant full length protein (Human), expressed in Escherichia coli. |
| Specificity: | In immunoblotting, the antibody shows no cross-reactivity with recombinanthuman VEGF, PDGFAA, PDGFAB, and PDGFBB. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Protein G purified Mouse ascites. |
| Uses: | ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 ul/well). WB: The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein. |
| Localization: | The three isoforms are secreted but PlGF2 appears to remain cell attached unless released by heparin. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | ELISA 2 ug/ml , Western Blot 1 - 2 ug/ml |
| Species Summary: | Hu, |
| Alternate Names: | Anti-PGF antibody; Anti-PIGF1 antibody; Anti-placental growth factor antibody; Anti-Placental growth factor vascular endothelial growth factor related protein antibody; Anti-Placental growth factor vascular endothelial growth factor-related antibody; Anti-PlGF 2 antibody; Anti-PLGF antibody; Anti-PlGF2 antibody; Anti-Vascular endothelial growth factor related protein antibody |
| Package Size: | 0.1 mg |
| Notes Main: | Do not store in frost-free freezer. Endotoxin level is <10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. |
| General References: | General / background references:DiPalma T et al. The placenta growth factor gene of the mouse. Mamm Genome 7:6-12 (1996). Maglione D et al. Two alternative mRNAs coding for the angiogenic factor, placenta growth factor (PlGF), are transcribed from a single gene of chromosome 14. Oncogene 8:925-31 (1993). Kaipainen A et al. The related FLT4, FLT1, and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells. J Exp Med 178:2077-88 (1993). Maglione D et al. Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor. Proc Natl Acad Sci U S A 88:9267-71 (1991). |
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