| CatalogCode: | | NB120-10678 |
| ProductName: | | HGF Antibody |
| Product Description: | | Mouse Monoclonal anti-HGF (24612.111) |
| Clone: | | 24612.11 |
| Clonality: | | Monoclonal |
| Immunogen: | | Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21. |
| Specificity: | | The product shows no cross reactivity with other tested cytokines using indirect ELISA. |
| CrossReactivity: | | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | | 0.1 mg Mouse ascites. |
| Uses: | | ELISA: detects <1.2 ng/well of rhHGF. WB: Use under non-reducing conditions. Predicted molecular weight: 83.1 kDa. Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22C. |
| Localization: | | Secreted |
| Background: | | Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF. |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | Constituents: PBS |
| ListPrice: | | 325 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-F-TCF antibody; anti-HGFB antibody; anti-HPTA antibody; anti-SF antibody |
| ProteinTarget: | | HGF |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | General / background references:Strain AJ Hepatocyte growth factor: another ubiquitous cytokine. J Endocrinol 137:1-5 (1993). Rubin JS et al. A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor. Proc Natl Acad Sci U S A 88:415-9 (1991). Stoker M et al. Scatter factor is a fibroblast-derived modulator of epithelial cell mobility. Nature 327:239-42 (1987). Nakamura T et al. Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures. Proc Natl Acad Sci U S A 83:6489-93 (1986). |
| NotesMain: | | Endotoxin: <5 ng/vial by LAL method. |
| more information: | | company product webpage |