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| Catalog Code: | NB120-10604 |
| Product Name: | Endostatin Antibody |
| Product Description: | Rat Monoclonal anti-Endostatin (91318) |
| Clone: | 91318 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Purified recombinant mouse endostatin, expressed in insect Sf 21 cells. |
| Cross Reactivity: | Cross-reacts with Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Affinity purified Rat ascites. |
| Uses: | ELISA: Use at a concentration of 0.5 - 1 ug/ml. The detection limit for recombinant mouse endostatin is approximately 6 ng/well. WB: Use at a concentration of 1 - 2 ug/ml. The detection limit for recombinant mouse endostatin is approximately 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Predicted molecular weight: 22 kDa. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. |
| Storage: | Store at 4 degrees short term and -20 degrees long term. |
| Purity: | Affinity purified |
| Isotype: | IgG2b |
| Host Name: | Rat |
| Buffer: | PBS |
| Application Summary: | ELISA, Western Blot |
| Species Summary: | Mu, |
| Package Size: | 0.1 mg |
| Preservative: | No Preservative |
| Notes Main: | Endotoxin level is <10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. |
| General References: | General / background references:Dixelius J et al. Endostatin regulates endothelial cell adhesion and cytoskeletal organization. Cancer Res 62:1944-7 (2002). Bloch W et al. The angiogenesis inhibitor endostatin impairs blood vessel maturation during wound healing. FASEB J 14:2373-6 (2000). Sasaki T et al. Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity. J Mol Biol 301:1179-90 (2000). O'Reilly MS et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88:277-85 (1997). |
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