| CatalogCode: | NB120-10536 |
| ProductName: | Cdc14A Antibody |
| Product Description: | Mouse Monoclonal anti-Cdc14A (DCS-291) |
| Clone: | DCS-291 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant fragment (Human). |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 ug/ml. Detects a band of approximately 50 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53. |
| Storage: | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA |
| ListPrice: | 285 |
| AppSummary: | ELISA, ICC, IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CDC 10 antibody, anti-Cdc 14 antibody, anti-Cdc 14A antibody, anti-Cdc 14A1 antibody, anti-Cdc 14A2 antibody, anti-CDC10 antibody, anti-Cdc14 antibody, anti-CDC14 cell division cycle 14 homolog A antibody, anti-CDC14 homolog A antibody, anti-Cdc14A1 antibody, anti-Cdc14A2 antibody, anti-Cell Division Cycle 14 Homolog A antibody, anti-Dual specificity protein phosphatase CDC 14A antibody, anti-Dual specificity protein phosphatase CDC14A antibody, anti-hCDC 14 antibody, anti-hCDC14 antibody |
| ProteinTarget: | Cdc14A |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Mailand N et al. Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nat Cell Biol 4:317-22 (2002). Kaiser BK et al. Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdc14A phosphatase. Mol Biol Cell 13:2289-300 (2002). Li L et al. A family of putative tumor suppressors is structurally and functionally conserved in humans and yeast. J Biol Chem 272:29403-6 (1997). |
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