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| Catalog Code: | NB120-10533 |
| Product Name: | Cdc2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdc2 [Tyr15] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide (Human) conjugated to KLH. |
| Epitope: | anti-Cdc 2 antibody, anti-CDK 1 antibody, anti-CDK1 antibody, anti-Cell division control protein 2 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell division cycle 2 G1 to S and G2 to M antibody, anti-Cell Divsion Cycle 2 Protein |
| Specificity: | Anti-phospho-cdc2 (phosphotyrosine 15) detects cdc2 and cdk2 only when catalytically inactivated by phosphorylation at Tyr15. It does not react with non-phosphorylated cdc2, nor cdk4, cdk6 or cdk7. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.025 ml |
| Uses: | WB: Use at a dilution of 1/1000. Predicted molecular weight: 34 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration. |
| Storage: | Store at 0° to −20 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: None Constituents: 50% Glycerol, 100ug/ml BSA, 150mM Sodium Chloride, 10mM HEPES. pH |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-CDK1 antibody, anti-DKFZp686L20222 antibody, anti-MGC111195 antibody |
| Package Size: | 0.025 ml |
| Phosphorylated: | Tyr15 |
| General References: | General / background references:Galaktionov K et al. Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev 9:1046-58 (1995). Atherton-Fessler S et al. Mechanisms of p34cdc2 regulation. Mol Cell Biol 13:1675-85 (1993). Norbury C & Nurse P Animal cell cycles and their control. Annu Rev Biochem 61:441-70 (1992). |
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more information: | company product webpage |