| CatalogCode: | | NB120-10524 |
| ProductName: | | DAP Kinase 1 Antibody |
| Product Description: | | Mouse Monoclonal anti-DAP Kinase 1 (DKPS308) [Ser308] |
| Clone: | | DKPS308 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1. |
| CrossReactivity: | | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | | 0.5 ml |
| Uses: | | Dot: Use at a concentration of 1 - 2 ug/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector.ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic |
| Background: | | DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death. DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy. |
| Storage: | | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | Preservative: 15mM Sodium Azide Constituents: 0.01mM PBS. pH 7.4 |
| ListPrice: | | 285 |
| AppSummary: | | WB, ELISA, IP |
| ALTnames: | | anti-DAK1 antibody, anti-DAP K1 antibody, anti-DAPK 1 antibody, anti-DAPK antibody, anti-DAPK1 antibody, anti-Death Associated Protein Kinase 1 antibody, anti-DKFZp781I035 antibody |
| ProteinTarget: | | DAP Kinase 1 (phospho S308) |
| PackageSize: | | 0.05 ml |
| GeneralRef: | | General / background references:Cohen O et al. DAP-kinase participates in TNF-alpha- and Fas-induced apoptosis and its function requires the death domain. J Cell Biol 146:141-8 (1999). Cohen O et al. DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity. EMBO J 16:998-1008 (1997). Deiss LP et al. Cathepsin D protease mediates programmed cell death induced by interferon-gamma, Fas/APO-1 and TNF-alpha. EMBO J 15:3861-70 (1996). |
| Phosphorylated: | | Ser308 |
| more information: | | company product webpage |