| CatalogCode: | NB120-10520 |
| ProductName: | Bad Antibody |
| Product Description: | Rat Monoclonal anti-Bad (64103) |
| Clone: | 64103 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant N-Termanal fragment (Mouse). |
| Specificity: | This antibody will also detect a 32 kDa band that comigrates with recombinant Bad on immunoblots. |
| CrossReactivity: | Cross-reacts with Mouse.Does not cross-react with Human.Not yet tested in other species. |
| Packaging: | 0.5 mg |
| Uses: | Dot: a working antibody concentration of approximately 1 ug/ml is recommended using extracts from 2 x 10<sup>6</sup> mouse cytotoxic T cell line (CTLL2) or L929 fibroblast cells in an enhanced chemiluminescent (ECL) assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Mitochondrial |
| Background: | Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Isotype: | IgG2a |
| Host_Name: | Rat |
| Buffer: | Preservative: None Constituents: PBS |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Rt, Hu(-) |
| ALTnames: | anti-Bcl-2 binding component 6 antibody, anti-BBC 2 antibody, anti-BBC2 antibody, anti-BBC6 antibody, anti-Bcl 2 Antagonist of Cell Death antibody, anti-Bcl 2 Binding Component 6 antibody, anti-BCL X / BCL 2 Binding Protein antibody, anti-BCL X Binding Protein antibody, anti-Bcl XL/Bcl 2 Associated Death Promoter antibody, anti-Bcl2 Associated Death Promoter antibody, anti-Bcl2 Like 8 Protein antibody, anti-BCL2L8 antibody, anti-BclXL antibody, anti-Proapoptotic BH3 Only Protein antibody |
| ProteinTarget: | Bad |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Datta SR et al. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91:231-41 (1997). Zha J et al. Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L) Cell 87:619-28 (1996). Oltvai ZN et al. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-19 (1993). |