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| Catalog Code: | NB120-10454 |
| Product Name: | Caspase 11 Antibody |
| Product Description: | Rat Monoclonal anti-Caspase 11 (17D9) |
| Clone: | 17D9 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | A recombinant p30 subunit of Mouse Caspase 11. |
| Cross Reactivity: | Cross-reacts with Mouse.Expected to cross-react with Rat due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 5 - 10 ug/ml (determined by using a whole cell extract of mouse spleen stimulated with lipopolysaccharide (LPS)). The antibody may be used for immunoblotting to detect the full length caspase 11 (apparent size of 35-43 kDa), and the p10 and p30 fragments.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Caspase 11 (also known as ICH3) gene encodes two protein products of 38 and 43 kDa that belong to the Ced3/ICE family of cysteine proteases. Caspase 11 is an upstream caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits. Monoclonal antibodies reacting specifically with caspase 11 are useful tools for the study of the protease network, involved in development and regulation, governing the life and death of cells and tissues. |
| Isotype: | IgG2a |
| Host Name: | Rat |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Application Summary: | immunohistochemistry-Fr, immunoprecipitation, Western Blot |
| Species Summary: | Mu |
| Alternate Names: | anti-Casp 4 antibody, anti-CASP11 antibody, anti-Casp4 antibody, anti-Caspase 11 apoptosis related cysteine protease antibody, anti-Caspase 4 antibody, anti-Caspase 4 apoptosis related cysteine peptidase antibody, anti-Caspase11 antibody, anti-Caspase4 antibody, anti-CASPL antibody, anti-Ich 3 antibody, anti-Ich3 antibody, anti-ICH3 protease antibody |
| Package Size: | 0.1 ml |
| Notes Main: | For continuous use, store at 2-8 degrees C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| General References: | General / background references:Wang S et al. Murine caspase-11, an ICE-interacting protease, is essential for the activation of ICE. Cell 92:501-9 (1998). Thornberry NA & Lazebnik Y Caspases: enemies within. Science 281:1312-6 (1998). Wang S et al. Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE. J Biol Chem 271:20580-7 (1996). |
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