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| Catalog Code: | NB120-10450 |
| Product Name: | Caspase 8 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase 8 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: SDSPREQDSESQTLDK conjugated to KLH, corresponding to N terminal amino acids 209-224 of Human Caspase 8. |
| Epitope: | The immunogen sequence corresponds to the N-terminal region of the enzyme p20 subunit and is identical in human caspase 8 isoforms (caspase8b/MACH alpha 2, MACH beta 3 and MACH beta 4). |
| Specificity: | Anti-Caspase 8 (SK-16) recognizes human caspase 8a and caspase 8b by immunoblotting. The product may also detect the caspase 8 MACH beta 3/beta 4 isoforms. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (85% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a dilution of 1/1000 (determined by using a whole cell extract of the human Burkitt lymphoma Raji cell line). Expected bands at: 55 kDa (human caspase 8a) and 54 kDa (caspase 8b). Possible bands at 30-32 kDa (caspase 8 MACH beta 3/beta 4 isoforms). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 0.01M PBS. pH 7.4 |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic cysteine protease antibody, anti-Apoptotic protease Mch 5 antibody, anti-CAP 4 antibody, anti-CAP4 antibody, anti-CASP 8 antibody, anti-CASP8 antibody, anti-Caspase8 antibody, anti-CED 3 antibody, anti-FADD homologous ICE/CED 3 like protease antibody, anti-FADD Homologous ICE/CED3 Like Protease antibody, anti-FADD Like ICE antibody, anti-FLICE antibody, anti-ICE like apoptotic protease 5 antibody, anti-MACH antibody, anti-MCH 5 antibody, anti-MCH5 antibody, anti-MGC78473 antibody, anti-MORT1 associated CED 3 homolog antibody, anti-MORT1 associated CED3 homolog antibody |
| Package Size: | 0.1 ml |
| Notes Main: | For continuous use, store at 2-8 degrees C for up to one month.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| General References: | General / background references:Martin DA et al. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal. J Biol Chem 273:4345-9 (1998). Scaffidi C et al. FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b. J Biol Chem 272:26953-8 (1997). Hu S et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 272:17255-7 (1997). Nicholson DW & Thornberry NA Caspases: killer proteases. Trends Biochem Sci 22:299-306 (1997). Cohen GM Caspases: the executioners of apoptosis. Biochem J 326 ( Pt 1):1-16 (1997). Muzio M et al. FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. J Biol Chem 272:2952-6 (1997). Muzio M et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex. Cell 85:817-27 (1996). Boldin MP et al. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death. Cell 85:803-15 (1996). Fernandes-Alnemri T et al. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc Natl Acad Sci U S A 93:7464-9 (1996). Srinivasula SM et al. Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc Natl Acad Sci U S A 93:14486-91 (1996). |
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