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| Catalog Code: | NB120-10449 |
| Product Name: | Caspase 7 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase 7 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: KPDRSSFVPSLFSKKKKN-C conjugated to maleimide-activated BSA, corresponding to amino acids 25-42 of Human Caspase 7 with C-terminal added cysteine. |
| Epitope: | Located at the N-terminal region of caspase 7 (amino acid residues 25-42 with C-terminal added cysteine). The epitope also corresponds to amino acid residues 2-19 of the human enzyme p20 subunit. |
| Specificity: | The antibody reacts specifically with procaspase 7 (Mch3). |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (82% identity with immunogen) and Rat (85% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a dilution of 1/1000 (determined by using a whole extract of a Jurkat human T-cell leukemia cell line). Predicted molecular weight: 35 kDa. An additional weak band, representing a caspase 7 degradation product, may be detected in some cell line extracts.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Apoptosis related cysteine peptidase antibody, anti-Apoptosis Related Cysteine Protease antibody, anti-Apoptotic Protease MCH3 antibody, anti-CASP 7 antibody, anti-CMH1 antibody, anti-ICE LAP3 antibody, anti-ICE like Apoptotic Protease 3 antibody, anti-MCH3 antibody |
| Package Size: | 0.1 ml |
| Notes Main: | For continuous use, store at 2-8 degrees C for up to one month.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| General References: | General / background references:Bossy-Wetzel E & Green DR Caspases induce cytochrome c release from mitochondria by activating cytosolic factors. J Biol Chem 274:17484-90 (1999). Chandler JM et al. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver. J Biol Chem 273:10815-8 (1998). Kidd VJ Proteolytic activities that mediate apoptosis. Annu Rev Physiol 60:533-73 (1998). Cryns V & Yuan J Proteases to die for. Genes Dev 12:1551-70 (1998). Duan H et al. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J Biol Chem 271:1621-5 (1996). Lippke JA et al. Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32. J Biol Chem 271:1825-8 (1996). Fernandes-Alnemri T et al. Mch3, a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res 55:6045-52 (1995). |
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