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| Catalog Code: | NB120-10438 |
| Product Name: | AKT1 Antibody |
| Product Description: | Mouse Monoclonal anti-AKT1 (PKB-175) |
| Clone: | PKB-175 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: DSERRPHFPQFSYSAS conjugated to BSA, corresponding to amino acids 461-477 of Human AKT1. |
| Specificity: | synthetic peptide corresponding to amino acids 461-477 of human PKBá/Akt1. The sequence is identical in mouse, rat, and bovine PKBá/Akt1, highly conserved in PKBâ/Akt2, and diverges in PKBã. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (93% identity with immunogen), Cow (100% identity with immunogen), Chicken (93% identity with immunogen) and Sheep (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | WB: Use at a dilution of 1/5000. Detects a band of approximately 56 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene). |
| Storage: | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 0.01M PBS. pH 7.4 |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-MGC99656 antibody, anti-PKB antibody, anti-PRKBA antibody, anti-RAC antibody, anti-RAC-ALPHA antibody |
| Package Size: | 0.05 ml |
| General References: | General / background references:Coffer PJ & Woodgett JR Molecular cloning and characterisation of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families. Eur J Biochem 201:475-81 (1991). Jones PF et al. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci U S A 88:4171-5 (1991). Bellacosa A et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science 254:274-7 (1991). |
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