| CatalogCode: | NB120-10437 |
| ProductName: | NFkB p65 Antibody |
| Product Description: | Mouse Monoclonal anti-NFkB p65 (NF-12) |
| Clone: | NF-12 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant, mouse NF-kB p65 fragment (C-terminal 151 amino acids). |
| Specificity: | This antibody recognizes an epitope within the C-terminal region of the mouse NF-kappaB p65. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.05 ml Ascites Mouse ascites. |
| Uses: | ICC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa. A minimum working dilution of 1/1000 is determined by immunoblotting using a whole cell extract of 3T3 mouse fibroblast cells.Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer. |
| Storage: | tore at 4C short term. Aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Raw Ascites |
| ListPrice: | 325 |
| NovusRef: | 1. Chou S-Y, Weng J-Y, Lai H-L, et al. Expanded-Polyglutamine Huntingtin Protein Suppresses the Secretion and Production of a Chemokine (CCL5/RANTES) by Astrocytes. J Neurosci. March 26, 2008;28(13):3277-90. |
| AppSummary: | WB, ICC |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | Anti-MGC131774 antibody, Anti-NFKB 3 antibody, Anti-NFKB3 antibody, Anti-Nuclear Factor NF Kappa B p65 Subunit antibody, Anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, Anti-RELA antibody, Anti-Transcription Factor p65 antibody, Anti-V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody, Anti-v-rel reticuloendotheliosis viral oncogene homolog A antibody |
| ProteinTarget: | NFkB p65 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:May MJ & Ghosh S Signal transduction through NF-kappa B. Immunol Today 19:80-8 (1998). |
|