| CatalogCode: | NB120-10434 |
| ProductName: | DNase I Antibody |
| Product Description: | Rabbit Polyclonal anti-DNase I |
| Clonality: | Polyclonal |
| Immunogen: | Full length native protein (purified) (Cow). |
| CrossReactivity: | Cross-reacts with Cow. Not yet tested in other species. |
| Packaging: | 0.5 mg |
| Uses: | WB: Use at a concentration of 1 ug/ml. Detects a band of approximately 33 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Deoxyribonuclease I (DNase I) is a Ca2+ and Mg2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. Deoxyribonuclease I (DNase I) is an endonuclease that hydrolyzes double-stranded or single-stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5' phosphate mononucleotides and oligonucleotides. In the presence of magnesium ion, DNase I attacks each strand of DNA independently and the cleavage sites are random. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.01% Sodium Azide Constituents: PBS. pH 7.2 |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Bv |
| ALTnames: | anti-Dornase alfa antibody, anti-Deoxyribonuclease 1 antibody, anti-Deoxyribonuclease I antibody, anti-Deoxyribonuclease1 antibody, anti-DNASE 1 antibody, anti-DNase I lysosomal antibody, anti-DNASE1 antibody, anti-DNaseI antibody, anti-DNL1 antibody, anti-Dornase alfa antibody, anti-DRNI antibody, anti-FLJ38093 antibody, anti-Human urine deoxyribonuclease I antibody |
| ProteinTarget: | DNase I |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Shak S et al. Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum. Proc Natl Acad Sci U S A 87:9188-92 (1990). Arends MJ et al. Apoptosis. The role of the endonuclease. Am J Pathol 136:593-608 (1990). Wyllie AH Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555-6 (1980). |