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| Catalog Code: | NB120-10420 |
| Product Name: | FGF2 Antibody |
| Product Description: | Rabbit Polyclonal anti-FGF2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | Does not cross react with recombinant mouse and human IL6, recombinant human IL1 alpha and IL1 beta, recombinant human TNF alpha, recombinant human IL3, and recombinant human FGF acidic. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. RIA Characterization: The antiserum is characterized utilizing a second antibody-polyethylene glycol (PEG) RIA protocol, where 0.1 ml of a minimum 1/10000 dilution of antiserum has been found to bind at least 40% of 100 picograms of iodinated recombinant human bFGF. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = minimum 1.0 x 109 L/mole.An minimum antibody concentration of 5 g/ml neutralizes 50% of the biological activity of 1 unit of bFGF using the mouse fibroblast cell line 3T3.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Background: | FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: 0.1% Sodium Azide Constituents: 0.01M PBS. pH 7.4 |
| Application Summary: | ELISA , Western Blot , Immunoassay 1:10,000, RID, Other |
| Species Summary: | Hu |
| Alternate Names: | anti-BFGF antibody, anti-FGFB antibody, anti-HBGH-2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% Sodium Azide |
| General References: | General / background references:Gospodarowicz D et al. Structural characterization and biological functions of fibroblast growth factor. Endocr Rev 8:95-114 (1987). Esch F et al. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci U S A 82:6507-11 (1985). Gospodarowicz D Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249:123-7 (1974). |
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