| CatalogCode: | NB120-10400 |
| ProductName: | CXCR1 Antibody |
| Product Description: | Mouse Monoclonal anti-CXCR1 (42705.111) |
| Clone: | 42705.11 |
| Clonality: | Monoclonal |
| Immunogen: | Human CXCR1 transfected NSO mouse myeloma cells. |
| Specificity: | Human CXCR1 reacts with CXCR1 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR2.The antiboody, in the presence of anti-CXCR2 (Sigma Product No. C6348), will neutralize human cell surface CXCR-1 mediated-myloperoxidase release from human granulocytes induced by IL-8. The antibody reacts with human CXCR1 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CXCR2. |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse IgG. |
| Uses: | FACS: Use 10ul for 10<sup>5-6</sup>human blood cells.Inhib: For neutralization, a working concentration of 0.1-0.3 u g/ ml of CXCR1 (in the presence of 10 u g/ ml CXCR2, will block 50% of the bioactivity due to 1 ug/ml recombinant human IL8 in an assay measuring myeloperoxidase released from human granulocytes. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Background: | Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR1 (IL8RA, or type I IL8 receptor) and CXCR2 (IL8RB, or type II IL8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca 2+ levels. Whereas CXCR1 is highly specific for IL8, CXCR2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GROs alpha, beta and gamma and NAP-2, and ENA-78. In contrast, PF4 and IP10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR2. CXCR1 and CXCR2 are expressed by neutrophils but not B lymphocytes or T lymphocytes. |
| Purity: | protein G purified |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | Preservative: None Constituents: PBS Endotoxin level is < 10 ng per mg antibody as determined b |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Hu |
| ALTnames: | anti-C C antibody, anti-C C CKR 1 antibody, anti-CCCKR1 antibody, anti-CD 181 antibody, anti-CD128 antibody, anti-CD181 antibody, anti-CD181 antigen antibody, anti-CDw128a antibody, anti-Chemokine (C X C motif) receptor 1 antibody, anti-Chemokine (C X C) receptor 1 antibody, anti-Chemokine receptor 1 antibody, anti-CKR 1 antibody, anti-CMKAR1 antibody, anti-CXCR 1 antibody, anti-High affinity interleukin 8 receptor A antibody, anti-IL 8 receptor type 1 antibody, anti-IL 8R A antibody, anti-IL8 receptor antibody, anti-IL8 receptor type 1 antibody, anti-IL8R1 antibody, anti-IL8RA antibody, anti-IL8RBA antibody, anti-Interleukin 8 receptor alpha antibody, anti-Interleukin 8 receptor type 1 antibody, anti-Interleukin 8 receptor type A antibody |
| ProteinTarget: | CXCR1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). Moser B et al. Expression of transcripts for two interleukin 8 receptors in human phagocytes, lymphocytes and melanoma cells. Biochem J 294-pt1:285-92 (1993). Murphy PM & Tiffany HL Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253:1280-3 (1991). |
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