| CatalogCode: | NB120-10392 |
| ProductName: | MCP2 Antibody |
| Product Description: | Goat Polyclonal anti-MCP2 |
| Clonality: | Polyclonal |
| Immunogen: | Purified E. coli-derived recombinant human MCP2. |
| Specificity: | Will not neutralize the biological activity of mouse JE/MCP1, human MCP1 or human MCP3. Based on direct ELISA and immunoblotting results (non-reducing conditions), shows less than 40% cross-reactivity with human HCC1, less than 10% cross reactivity with human eotaxin and less than 5% cross-reactivity with human MCP1. Shows no crossreactivity with other cytokines tested using direct ELISA.The antibody may be used for neutralization, localization and detection of human MCP-2. It will not neutralize recombinant human MCP-1 or MCP-3, or recombinant mouse JE. The antibody shows <40% cross-reactivity with recombinant human HCC-1, <10% cross-reactivity with recombinant human eotaxin, and <5% cross-reactivity with recombinant human MCP-1. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.025 mg |
| Uses: | ELISA: Use at a concentration of 0.5 - 1 ug/ml to detect approximately 0.3 ng/well of recombinant human MCP2.Neut: Use at a concentration of 2 - 5 ug/ml. WB: Use at a concentration of 0.1 - 0.2 ug/ml. The detection limit is approximately 20ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 13kDa. Neutralization Dose 50 (ND50) is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is approximately 2 - 5 ug/ml in the presence of 0.4 ug/ml of human MCP2, using the human monocyte chemotaxis assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Background: | MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Preservative: NoneConstituents: PBS |
| ListPrice: | 285 |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CCL 8 antibody, anti-CCL8 antibody, anti-Chemokine CC Motif Ligand 8 antibody, anti-HC14 antibody, anti-Monocyte Chemotactic Protein 2 antibody, anti-SCYA10 antibody, anti-SCYA8 antibody, anti-Small inducible cytokine A8 [Precursor] antibody |
| ProteinTarget: | MCP2 |
| PackageSize: | 0.025 mg |
| GeneralRef: | General / background references:Weber M et al. Monocyte chemotactic protein MCP-2 activates human basophil and eosinophil leukocytes similar to MCP-3. J Immunol 154:4166-72 (1995). Van Damme J et al. Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family. J Exp Med 176:59-65 (1992). |
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