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| Catalog Code: | NB120-10391 |
| Product Name: | MCP2 Antibody |
| Product Description: | Mouse Monoclonal anti-MCP2 (35509.11) |
| Clone: | 35509.11 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli. |
| Specificity: | By ELISA, the antibody shows < 20% cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. In addition, the antibody does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, rhIL8, rhIP10, rhMIP1 alpha, rhMIP1 beta, rmMIP1 beta, rhMCP3, rhRANTES. Neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1á. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse IgG. |
| Uses: | ELISA: Use at a concentration of 0.5 - 1 ug/ml to detect <12.5ng/ml of recombinant human MCP2.Neut: Use at a concentration of 0.5 - 10 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa. Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22 |
| Localization: | Secreted |
| Background: | MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Preservative: None Constituents: PBS |
| Application Summary: | ELISA, Western Blot, Other |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-CCL 8 antibody, anti-CCL8 antibody, anti-Chemokine CC Motif Ligand 8 antibody, anti-HC14 antibody, anti-Monocyte Chemotactic Protein 2 antibody, anti-SCYA10 antibody, anti-SCYA8 antibody, anti-Small inducible cytokine A8 [Precursor] antibody |
| Package Size: | 0.1 mg |
| Notes Main: | Endotoxin: < 5 ng/vial by LAL method. |
| General References: | General / background references:Matsushima K et al. Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J Exp Med 169:1485-90 (1989). |
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