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| Catalog Code: | NB120-10383 |
| Product Name: | Macrophage Inflammatory Protein 1 beta Antibody |
| Product Description: | Goat Polyclonal anti-Macrophage Inflammatory Protein 1 beta |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1 mg/ml |
| Immunogen: | Purified insect cell line Sf21-derived recombinant human MIP-1beta. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Protein G purified Goat antisera. |
| Uses: | ELISA: The detection limit is approximately 0.16 ng/well.IHC: Use at a concentration of 0.5 - 5 ug/ml to detect MIP-1beta in cultured cells or tissue sections. WB: The detection limit is approximately 1 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 11 kDa.Neutralization Dose (ND50) is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is approximately 0.5 - 2 ug/ml in the presence of 0.1 ug/ml of human MIP-1beta, using the human monocyte chemotaxis assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Background: | MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD-5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Constiuents: PBS |
| Application Summary: | ELISA 0.5 - 1 ug/ml, immunohistochemistry 0.5 - 5 ug/ml, Western Blot 0.1 -0. 2 ug/ml, Neut: 0.5 - 2 mg/ml |
| Species Summary: | Hu, |
| Alternate Names: | Anti-ACT2 antibody, Anti-CCL4 antibody, Anti-ccl4l 1 antibody, Anti-CCL4L1 antibody, Anti-Chemokine CC Motif Ligand 4 antibody, Anti-G 26 T lymphocyte secreted protein antibody, Anti-HC21 antibody, Anti-lag 1 antibody, Anti-LAG1 antibody, Anti-Lymphocyte activation gene 1 antibody, Anti-MIP1 beta antibody, Anti-Small inducible cytokine A4 antibody |
| Package Size: | 0.1 mg |
| General References: | General / background references:Leith JG et al. CD8+ T-cell-mediated suppression of HIV-1 long terminal repeat-driven gene expression is not modulated by the CC chemokines RANTES, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta. AIDS 11:575-80 (1997). Graham GJ et al. Identification and characterization of an inhibitor of haemopoietic stem cell proliferation. Nature 344:442-4 (1990). Lipes MA et al. Identification, cloning, and characterization of an immune activation gene. Proc Natl Acad Sci U S A 85:9704-8 (1988). |
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