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| Catalog Code: | NB120-10369 |
| Product Name: | Eotaxin Antibody |
| Product Description: | Goat Polyclonal anti-Eotaxin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.1 mg/ml |
| Immunogen: | Purified E. coli-derived recombinant human eotaxin |
| Specificity: | Eotaxin will neutralize the biological activity of mouse eotaxin at a 5-fold higher IgG concentration. Based on direct ELISA and immunoblotting results (non-reducing conditions), it shows less than 20% cross-reactivity with mouse eotaxin, less than 15% cross-reactivity with human MCP2 and human MCP3 and less than 5% cross-reactivity with human MCP1. This antibody shows no cross-reactivity with other cytokines tested using direct ELISA. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.025 mg |
| Uses: | Neut: Use at a concentration of 1 - 3 ug/ml. Neutralization Dose 50 (ND50) is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is approximately 1 - 3 ug/ml in the presence of 0.05 ug/ml of human eotaxin, using CCR3 transfected Y3 cells in a chemotaxis assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Background: | Originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. cDNA clones for guinea pig, mouse and human eotaxin were isolated. Mouse eotaxin cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 residues are cleaved to generate the 74 residue mature protein. Mouse eotaxin is approximately 60% identical to human eotaxin and guinea pig eotaxin. Mouse eotaxin shows high amino acid sequence identity to members of the MCP family. mRNA is expressed in a variety of tissues. Expression is induced in cultured endothelial cells in response to IFN-g. mRNA is also induced in response to the transplantation of IL-4- secreting tumor cells. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Preservative: None Constituents: PBS |
| Application Summary: | Functional Assay |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-CCL11 antibody, anti-Chemokine CC Motif Ligand 11 antibody, anti-Eosinophil Chemotactic Protein antibody, anti-MGC22554 antibody, anti-SCYA11 antibody, anti-Small inducible cytokine subfamily A member 11 antibody |
| Package Size: | 0.025 mg |
| General References: | General / background references:Garcia-Zepeda EA et al. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat Med 2:449-56 (1996). Ponath PD et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 97:604-12 (1996). Rothenberg ME et al. Murine eotaxin: an eosinophil chemoattractant inducible in endothelial cells and in interleukin 4-induced tumor suppression. Proc Natl Acad Sci U S A 92:8960-4 (1995). |
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