| CatalogCode: | NB120-10305 |
| ProductName: | Gemin 3 Antibody |
| Product Description: | Mouse Monoclonal anti-Gemin 3 (12H12) |
| Clone: | 12H12 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant domain of Gemin 3 protein corresponding to amino acids 368-548. |
| Specificity: | Detects Gemin 3 in human, bovine, hamster and chicken. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Cow an Chicken. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Mouse ascites. |
| Background: | Spinal muscular atrophy (SMA) is caused by reduced expression or mutations in the Survival of Motor Neurons (SMN) protein. Two copies of the SMN gene (SMN1 and SMN2) exist in humans on chromosome 5. Deletion or mutations in the telomeric copy (SMN1) cause the SMA phenotype. The severity of SMA is in direct correlation with the expression level of the SMN protein either from the SMN1 gene or a different spliced form of SMN from the SMN2 gene.2-3 The SMN protein forms a multi-protein complex with the Gemin proteins. Gemin2, 3, 5, and 7 interact directly with the SMN protein while the interaction of Gemin4 and 6 requires Gemin3 and 7 respectively. The SMN complex interacts with various protein substrates such as Sm and Lsm proteins of the spliceosomal snRNPs, fibrillarin, GAR1, RNA helicase A, the human hnRNP proteins (hnRNPQ, U, and R), coilin, and p53. Arginine and glycine rich regions in the protein substrates are important for the interaction with the SMN complex. Furthermore, methylation of arginines in these regions by the methylosome (aka PRTM5) enhances their affinity for the SMN. The SMN complex is important in various biological events such as assembly and restructuring of spliceosomal snRNPs, pre mRNA splicing and transcription.1, 4, 5 Monoclonal antibodies specific for Gemin3 are an important tool for studying the role of the Gemin protein family in nuclear processes. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 0.01 M PBS, pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | ELISA, IP, WB, ICC |
| SpeciesSummary: | Bv, Ch, Hu, Mu |
| ALTnames: | Anti-Component of gems 3 antibody; Anti-DDX 20 antibody; Anti-DDX20 antibody; Anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody; Anti-DEAD box protein 20 antibody; Anti-DEAD-box protein DP103 antibody; Anti-DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody; Anti-DEAD/H BOX 20 antibody; Anti-DKFZP434H052 antibody; Anti-DP103 antibody; Anti-Gemin3 antibody; Anti-Probable ATP-dependent RNA helicase DDX20 antibody; Anti-SMN interacting protein antibody |
| ProteinTarget: | Gemin 3 |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Charroux, B., et al., J. Cell Biol., 147, 1181-1193 (1999). 2. Paushkin, S., et al., Curr. Opin. Cell Biol., 14, 305-312 (2002). 3. Pellizzoni, L., et al., Proc. Natl. Acad. Sci. USA, 96, 11167-11172 (1999). 4. Baccon, J., et al., J. Biol. Chem., 277, 31957-31962 (2002). 5. Pellizzoni, L., et al., Science, 298, 1775-1779 (2002). |
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