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| Catalog Code: | NB120-10223 |
| Product Name: | cardiac Troponin T Antibody |
| Product Description: | Mouse Monoclonal anti-cardiac Troponin T (7G7) |
| Clone: | 7G7 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Full length protein (Human). |
| Epitope: | 60-70 aa |
| Specificity: | Does not cross-react with skeletal muscle troponin T, cTnT or TnC. |
| Cross Reactivity: | Cross-reacts with Human and Cow.Does not cross-react with Mouse, Rat, Cat, Dog, Fish, Goat, Pig or Rabbit.Not yet tested in other species. |
| Packaging: | 0.2 mg protein A purified Mouse IgG. |
| Uses: | AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 35.4 kDa. Can be paired for ELISA with <ab10216>Mouse monoclonal [2F3] to cardiac Troponin T</ab>, <ab8295>Mouse monoclonal [1C11] to cardiac Troponin T</ab>, <ab10220>Mouse monoclonal [6G9] to cardiac Troponin T</ab> and <ab8296>Mouse monoclonal [9G6] to cardiac Troponin T</ab>. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms. |
| Storage: | + 4 °C |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.4 |
| Application Summary: | immunoprecipitation, ELISA, Western Blot, Other |
| Species Summary: | Mu, |
| Package Size: | 0.2 mg |
| Preservative: | 0.1% Sodium Azide |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone: 7G7-9G6 For Sandwich ELISA pair this antibody with: NB120-10220 |
| General References: | General / background references:Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). |
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