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| Catalog Code: | NB110-86974 |
| Product Name: | Smad2 Antibody |
| Product Description: | Rabbit Polyclonal anti-Smad2 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.55 mg/ml |
| Immunogen: | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein. |
| Specificity: | This antibody is directed against human Smad2 protein. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence. The antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, and Rat. |
| Packaging: | 0.1 mg Affinity purified Rabbit antisera. |
| Uses: | This antibody has been tested for use in ELISA and western blotting. Expect a band approximately 52 kDa in size corresponding to Smad2 protein. |
| Background: | Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Application Summary: | ELISA 1:100000, immunohistochemistry User Optimized, Western Blot 1:1000-1:3000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-hMAD 2 antibody, anti-hSMAD2 antibody, anti-JV18 1 antibody, anti-JV18 antibody, anti-JV181 antibody, anti-MAD antibody, anti-MAD Related Protein 2 antibody, anti-MADH2 antibody, anti-MADR2 antibody, anti-MGC22139 antibody, anti-MGC34440 antibody, anti-Mothers Against Decapentaplegic Homolog 2 antibody, anti-mothers against DPP homolog 2 antibody, anti-SMAD 2 antibody, anti-SMAD antibody, anti-SMAD2 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.01% Sodium Azide |
| Homology: | Mouse 100% homology Rat 100% homology |
| General References: | 1. Yang,A.H., Huang,S.W., Chen,J.Y., er al. Leptin augments myofibroblastic conversion and fibrogenic activity of human peritoneal mesothelial cells: a functional implication for peritoneal fibrosis. Nephrol. Dial. Transplant. 2007; 22 (3), 756-762. 2. Liapis,G., Mylona,E., Alexandrou,P., et al. Effect of the different phosphorylated Smad2 protein localizations on the invasive breast carcinoma phenotype. APMIS 2007; 115 (2), 104-114. 3. Bruna,A., Darken,R.S., Rojo,F., et al. High TGFbeta-Smad activity confers poor prognosis in glioma patients and promotes cell proliferation depending on the methylation of the PDGF-B gene. Cancer Cell 2007; 11 (2), 147-160. 4. Peng,H., Shintani,S., Kim,Y. and Wong,D.T. Loss of p12CDK2-AP1 expression in human oral squamous cell carcinoma with disrupted transforming growth factor-beta-Smad signaling pathway. Neoplasia 2006; 8 (12), 1028-1036. 5. Olsen,J.V., Blagoev,B., Gnad,F., et al. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2006; 127 (3), 635-648. |
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