| request information: |
|
| Catalog Code: | NB110-8453 |
| Product Name: | cardiac Troponin I Antibody |
| Product Description: | Mouse Monoclonal anti-cardiac Troponin I (4C2) |
| Clone: | 4C2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Full length protein (Human). |
| Epitope: | 23-29 aa |
| Specificity: | reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I. |
| Cross Reactivity: | Cross-reacts with Mouse, Rat, Cow, Cat, Dog, Goat, Pig, Rabbit and Human.Not yet tested in other species. |
| Packaging: | 0.2 mg protein A purified Mouse ascites. |
| Uses: | ELISA, Immunoprecipitation, Western Blot, ImmunoassayPredicted molecular weight 24 kDa. These antibody combinations are recommended for sandwich ELISA, and demonstrate high sensitivity (0.02 ng/ml), good kinetics, low background and high reproducibility: <ab8289>Mouse monoclonal [19C7] to cardiac Troponin I</ab> can be used in combination with <ab10239>Mouse monoclonal [MF4] to cardiac Troponin I</ab>, or <ab10231>Mouse monoclonal [4C2] to cardiac Troponin I</ab> for capture, with <ab10226>Mouse monoclonal [16A11] to cardiac Troponin I</ab> and <ab8286>Mouse monoclonal [7B9] to cardiac Troponin C</ab> for detection, (specific for Troponin C).Can also be paired for ELISA with <ab8289>Mouse monoclonal [19C7] to cardiac Troponin I</ab> and <ab10226>Mouse monoclonal [16A11] to cardiac Troponin I</ab>. Using multiple combinations of MAbs antibodies with different epitope specificity as capture and multiple MAbs as detection, the sensitivity of the sandwich ELISA can be increased 2-4 fold. The ELISA based on these recommended Abs, recognize complexed and free forms of the antigen with same sensitivity. For better sensitivity and reproducibility for assay designing we recommend to use at least one antibody that recognizes the stable fragment of the cTnI molecule (30-110 aa section), as cTnI is proteolytically degraded in the blood stream.Because the amount of complexed cTnI in blood of AMI patients is very high, for cTnI immunodetection we recommend using also a MAb that is specific to TnC or a MAb specific to TnC together with MAb(s) specific to cTnI. Such approach helps improving the sensitivity and reproducibility of cTnI immunoassays. Optimal dilutions and concentrations should be determined by the end user. |
| Background: | Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. |
| Storage: | Store at 4 degrees C long term. |
| Purity: | protein A purified |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.4 |
| Application Summary: | ELISA assay dependent, immunoprecipitation assay dependent, Western Blot assay dependent, Immunoassay assay dependent |
| Species Summary: | Hu, |
| Alternate Names: | anti-CMH7 antibody, anti-cTnI antibody, anti-Familial hypertrophic cardiomyopathy 7 antibody, anti-MGC116817 antibody, anti-TNN I3 antibody, anti-TNNC 1 antibody, anti-TNNC1 antibody, anti-TNNI3 antibody, anti-Troponin I cardiac muscle antibody, anti-Troponin I type 3 cardiac antibody, anti-TroponinI antibody |
| Package Size: | 0.2 mg |
| Preservative: | 0.1% Sodium Azide |
| General References: | General / background references:Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). Cummins B et al. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:1333-44 (1987). |
order or
more information: | company product webpage |
|