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| Catalog Code: | NB110-8447 |
| Product Name: | cardiac Troponin I Antibody |
| Product Description: | Mouse Monoclonal anti-cardiac Troponin I (10B11) |
| Clone: | 10B11 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Full length protein (Human). |
| Epitope: | 16-20 aa. |
| Specificity: | Reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes dephospho- and phospho- forms of human cardiac Troponin I.Does not cross-react with skeletal muscle troponin I. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Cat, Dog, Pig and Rabbit.Does not cross-react with Mouse, Cow, Fish or Goat.Not yet tested in other species. |
| Packaging: | 0.2 mg |
| Uses: | immunoprecipitation, Western Blot, Immunoassay Predicted molecular weight 24 kDa. Optimal dilutions and concentrations should be determined by the end user. |
| Background: | cardiac Troponin I is phosphorylated in vivo and it can also be phosphorylated in vitro by cAMP-dependent protein kinase A. Sites of phosphorylation are the serines in the 22nd and 23rd position (Jaquet K et al). NB120-10225 recognizes the epitopes containing these two amino acid residues. |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.4 |
| Application Summary: | immunoprecipitation assay dependent, Western Blot assay dependent, Immunoassay assay dependent |
| Species Summary: | Hu |
| Alternate Names: | anti-CMH7 antibody, anti-cTnI antibody, anti-Familial hypertrophic cardiomyopathy 7 antibody, anti-MGC116817 antibody, anti-TNN I3 antibody, anti-TNNC 1 antibody, anti-TNNC1 antibody, anti-TNNI3 antibody, anti-Troponin I cardiac muscle antibody, anti-Troponin I type 3 cardiac antibody, anti-TroponinI antibody |
| Package Size: | 0.2 mg |
| Preservative: | 0.1% Sodium Azide |
| Notes Main: | Concentration varies from lot to lot and can be provided on request. |
| General References: | General / background references:Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). Jaquet K et al. Pattern formation on cardiac troponin I by consecutive phosphorylation and dephosphorylation. Eur J Biochem 231:486-90 (1995). Cummins B et al. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:1333-44 (1987). |
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