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| Catalog Code: | NB110-81742 |
| Product Name: | CD95 / Fas Antibody |
| Product Description: | Mouse Monoclonal anti-CD95 / Fas (LT95), conjugated to PE |
| Clone: | LT95 |
| Clonality: | Monoclonal |
| ListPrice: | 340 |
| Gene ID: | 10q24.1 |
| Reference Sequence: | 10q24.1 |
| Immunogen: | HUT-78 human T cell lymphoma cell line |
| Specificity: | The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. |
| Cross Reactivity: | Human. Not tested in other species. |
| Packaging: | 100 tests Mouse ascites. |
| Uses: | The reagent is designed for Flow Cytometry analysis of human blood cells using 20 mlreagent / 100 ml of whole blood or 10^6 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests. |
| Background: | CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses. |
| Storage: | Store in the dark at 2-8 C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored unde |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu, |
| Package Size: | 100 tests |
| Conjugate: | PE |
| Notes Main: | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. PE excitation: 488 nm, emission: 575 nm |
| General References: | 1. Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87. 2. Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16. 3. Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41. 4. Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63. 5. Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005 Jun 17;280(24):22856-67. Epub 2005 Mar 8. |
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