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| Catalog Code: | NB110-81697 |
| Product Name: | CD25 Antibody |
| Product Description: | Mouse Monoclonal anti-CD25 (MEM-140), conjugated to Biotin |
| Clone: | MEM-140 |
| Clonality: | Monoclonal |
| ListPrice: | 300 |
| Gene ID: | 10p15-p14 |
| Reference Sequence: | 10p15-p14 |
| Concentration: | 1 mg/ml |
| Immunogen: | PHA-activated peripheral blood leucocytes |
| Specificity: | The antibody MEM-140 reacts with CD25 (Interleukin-2 receptor a chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. HLDA VI; WS Code C C-54 |
| Cross Reactivity: | Human. Not tested in other species. |
| Packaging: | 0.1 mg Mouse ascites. |
| Uses: | The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. |
| Background: | CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. |
| Storage: | Store at 2-8C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20C. Avoid freeze/thaw cycles. |
| Isotype: | IgM |
| Host Name: | Mouse |
| Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu, |
| Package Size: | 0.1 mg |
| Conjugate: | Biotin |
| Notes Main: | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| General References: | 1. Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul;23(3):224-8. 2. Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75). Res Immunol. 1992 Jan;143(1):33-7. 3. Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14. 4. Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct;28(10):1319-25. 5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). |
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