| CatalogCode: | NB110-8146 |
| ProductName: | Fibrillin 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Fibrillin 1 (11C1.3) |
| Clone: | 11C1.3 |
| Clonality: | Monoclonal |
| Immunogen: | Microfibrils from zonular apparatus of bovine eye. |
| Epitope: | Amino acids 451 - 909. |
| CrossReactivity: | Cross-reacts with Human and Cow. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Electron Microscopy / Immunofluorescence / Immunohistochemistry: assay dependent. Western blot: use at a concentration of 1 - 2ug/ml for 2 hours at RT. By Western blot this detects a band of ~ 350 kDa, which corresponds to the predicted molecular weight of Fibrillin 1. |
| Localization: | Extracellular microfibrils. |
| Control: | Normal skin fibroblasts. |
| Background: | Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | 325 |
| AppSummary: | IF, IHC-Fr, WB |
| SpeciesSummary: | Bv, Hu |
| ProteinTarget: | Fibrillin 1 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Maier A et al. Fibrillin and elastin networks in extrafusal tissue and muscle spindles of bovine extraocular muscles. Invest Ophthalmol Vis Sci 35:3103-10 (1994). Wright DW et al. Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin. Matrix Biol 14:41-9 (1994). |