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| Catalog Code: | NB110-75107 |
| Product Name: | Ngfb Antibody |
| Product Description: | Rabbit Polyclonal anti-Ngfb |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | beta-NGF_MOUSE |
| Immunogen: | Native beta-nerve growth factor (Ngfb, beta-NGF) purified from mouse submandibular gland |
| Specificity: | This antiserum appears to be very specific for NGF. |
| Cross Reactivity: | Mouse, rat and human. Other species not yet tested. |
| Packaging: | 0.15 ml of Whole antisera antibody |
| Uses: | IHC, WB, inhibition of biological activity. Tested in vitro, this antibody abolishes the neuronal survival and the outgrowth actions of murine NGF in chicken DRG. A dilution of 1 : 500 to 1 : 4000 is recommended for IHC and WB; for inhibition of biological activities a dilution of up to 1 : 100 is recommended and for in vivo 1-20 µl per gram body weight. The optimal dilution should be determined by the end user. |
| Background: | NGF is a secreted molecule which plays an important roll in the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry 1:500 to 1:4000, Western Blot 1:500 to 1:4000 |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-Ngfb antibody, anti-beta-NGF antibody |
| Package Size: | 0.15 ml |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Manni L, et al. Neurosci. Lett. 426 (1), 39-44(2007). 2. Luo W, et al. Neuron 54 (5), 739-754(2007). 3. Arsenijevic D, et al. Eur. Cytokine Netw. 18 (2), 93-101(2007). 4. Ieda M, et al. Nat. Med. 13 (5), 604-612(2007). 5. Oakey R, et al. Immunogenetics 35 (4), 279-282(1992). |
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