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| Catalog Code: | NB110-74995 |
| Product Name: | TRPM4 Antibody |
| Product Description: | Sheep Polyclonal anti-TRPM4 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | TRPM4_RAT ; TRPM4_MOUSE |
| Immunogen: | A synthetic peptide as a part of rat and mouse transient receptor potential cation channel subfamily M member 4 (TRPM4) conjugated to immunogenic carrier protein was used as the immunogen. |
| Specificity: | Appears to be specific for TRPM4. |
| Cross Reactivity: | Rat, mouse. Other species not yet tested. |
| Packaging: | 0.1 ml of Whole antisera antibody |
| Uses: | IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user. |
| Background: | FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.; ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2).; SUBUNIT: Homomultimer.; SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Sheep |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry , Western Blot 1:300 to 1:4000 |
| Species Summary: | Rt, Mu |
| Alternate Names: | anti-Transient receptor potential cation channel subfamily M member 4 antibody, anti-Long transient receptor potential channel 4 antibody, anti-Calcium-activated non-selective cation channel 1 antibody, anti-Melastatin-like 2 antibody, anti-MLS2s antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Gibbs R.A, et al. Nature 428:493-521(2004). 2. Guinamard R, et al. J. Cardiovasc. Electrophysiol. 15:342-348(2004). 3. Guinamard R, et al. Hypertension 48:587-594(2006). 4. Murakami M, et al. Biochem. Biophys. Res. Commun. 307:522-528(2003). 5. Nilius B, et al. J. Biol. Chem. 278:30813-30820(2003). |
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