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| Catalog Code: | NB110-74821 |
| Product Name: | ATG5 Antibody |
| Product Description: | Rabbit Polyclonal anti-ATG5 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | ATG5_HUMAN ; ATG5_MOUSE ; ATG5_XENOPUS ; ATG5_CHICKEN |
| Immunogen: | A synthetic peptide as a part of human, mouse, chicken autophagy protein 5 (ATG5, ASP, APG5, APG5L, hAPG5) conjugated to an immunogenic carrier protein has been used as the immunogen. |
| Specificity: | Appears to be specific for ATG5. |
| Cross Reactivity: | Human, mouse, other species not yet tested. |
| Packaging: | 0.15 ml of Whole antisera antibody |
| Uses: | IHC, WB 1:300 to 1: 2000. The optimal dilution should be determined by the end user. |
| Background: | ATG5 is an important element for autophagy and may also play an important role in the apoptotic process. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. While the mRNA is ubiquitously transcribed its expression is highly upregulated in apoptotic cells. |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry , Western Blot 1:300 to 1:2000 |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-APG5-like antibody, anti-ATG5 antibody, anti-ASP antibody, anti-APG5 antibody, anti-APG5L antibody, anti-hAPG5 antibody, anti-apoptosis-specific protein antibody |
| Package Size: | 0.15 ml |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Ewing R.M, et al. Mol. Syst. Biol. 3, 89(2007). 2. Oh J.H, et al. Mamm. Genome 16 (12), 942-954(2005). 3. Pyo J.O, et al. J. Biol. Chem. 280 (21), 20722-20729(2005). 4. Suzuki Y, et al. Genome Res. 14 (9), 1711-1718(2004). 5. Simonsen A, et al. J. Cell. Sci. 117 (PT 18), 4239-4251(2004). 6. Mizushima N, et al. J. Cell Biol. 152 (4), 657-668(2001). 7. Schmeiser K, et al. Cytogenet. Cell Genet. 87 (3-4), 213-214(1999). 8. izushima N, et al. J. Biol. Chem. 273 (51), 33889-33892(1998). 9. Hammond E.M, et al. FEBS Lett. 425 (3), 391-395(1998). 10. Grand R.J, et al. Exp. Cell Res. 218 (2), 439-451(1995). |
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