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| Catalog Code: | NB110-74791 |
| Product Name: | Beclin-1 Antibody |
| Product Description: | Sheep Polyclonal anti-Beclin-1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | BECN1_HUMAN ; BECN1_RAT ; BECN1_MOUSE |
| Immunogen: | A synthetic peptide as a part of human, rat, mouse coiled-coil myosin-like BCL2-interacting protein (Beclin-1, BECN1, GT197, ATG6, VPS30) conjugated to immunogenic carrier protein has been used as the immunogen. |
| Specificity: | Appears to be specific for Beclin-1. |
| Cross Reactivity: | Human, mouse and rat. Other species not yet tested. |
| Packaging: | 0.5 mg of IgG purified antibody |
| Uses: | IHC, WB. Use at a concentration of 1-10 µg/ml. The optimal workiing concentration should be determined by the end user. |
| Background: | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in autophagy.; SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells; TISSUE SPECIFICITY: Ubiquitous |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Sheep |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry, Western Blot 1-10 µg/ml |
| Species Summary: | Hu, Mu, Rt |
| Alternate Names: | anti-Beclin-1 antibody, anti-BECN1 antibody, anti-GT197 antibody, anti-ATG6 antibody, anti-VPS30 antibody, anti-beclin1 antibody |
| Package Size: | 0.5 mg |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Feng W, et al. J. Mol. Biol. 372 (1), 223-235(2007). 2. Maiuri M.C, et al. Autophagy 3 (4), 374-376(2007). 3. Lee J.W, et al. APMIS 115 (6), 750-756(2007). 4. Maiuri M.C, et al. EMBO J. 26 (10), 2527-2539(2007). 5. Koneri K, et al. Anticancer Res. 27 (3B), 1453-1457(2007). 6. Kihara A, et al. EMBO Rep. 2 (4), 330-335(2001). 7. Liang X.H, et al. Nature 402 (6762), 672-676(1999). 8. Aita V.M, et al. Genomics 59 (1), 59-65(1999). 9. Liang X.H, et al. J. Virol. 72 (11), 8586-8596(1998). 10. Rommens J.M, et al. Genomics 28 (3), 530-542(1995). |
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