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| Catalog Code: | NB110-74732 |
| Product Name: | ASIC1 Antibody |
| Product Description: | Rabbit Polyclonal anti-ASIC1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | ASIC1_HUMAN ; ASIC1_RAT |
| Immunogen: | A synthetic peptide as a part of human and rat amiloride-sensitive cation channel 2, neuronal (ASIC1) conjugated to immunogenic carrier protein was used as the immunogen. |
| Specificity: | Appears to be specific for ASIC1. |
| Cross Reactivity: | Human and rat. Other species not yet tested. |
| Packaging: | 0.1 ml of Whole antisera antibody |
| Uses: | IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user. |
| Localization: | Cell Membrane; Multi-pass membrane protein Note: Localizes in synaptosomes at dendritic synapses of neurons. Co-localizes with DLG4. |
| Background: | This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown.; FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+).; SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons.; TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.; |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry, Western Blot 1:300 |
| Species Summary: | Hu, Rt. |
| Alternate Names: | anti-Acid-sensing ion channel 1 antibody, anti-ASIC1 antibody, anti-Brain sodium channel 2 antibody, anti-BNaC2 antibody, anti-ACCN2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Waldmann R, et al. Nature 386:173-177(1997). 2. Chen C.-C, et al. Proc. Natl. Acad. Sci. U.S.A. 95:10240-10245(1998). 3. Baessler E.-L, et al. J. Biol. Chem. 276:33782-33787(2001). 4. Ugawa S, et al. NeuroReport 12:2865-2869(2001). 5. Bassilana F, et al. J. Biol. Chem. 272:28819-28822(1997). 6. Escoubas P, et al. J. Biol. Chem. 275:25116-25121(2000). 7. Askwith C.C, et al. Neuron 26:133-141(2000). 8. Voilley N, et al. J. Neurosci. 21:8026-8033(2001). 9. Babini E, et al. J. Biol. Chem. 277:41597-41603(2002). 10. Coric T, et al. J. Biol. Chem. 278:45240-45247(2003). |
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