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| Catalog Code: | NB110-74722 |
| Product Name: | OPN4 Antibody |
| Product Description: | Rabbit Polyclonal anti-OPN4 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Omim ID: | OPN4_HUMAN |
| Immunogen: | A synthetic peptide as a part of human opsin-4 (OPN4, melanopsin, MOP) conjugated to immunogenic carrier protein was used as the immunogen. |
| Specificity: | Appears to be specific for opsin-4. |
| Cross Reactivity: | Human. Other species not yet tested. |
| Packaging: | 0.1 ml of Whole antisera antibody |
| Uses: | IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user. |
| Localization: | Membrane; Multi-pass membrane protein. Note=Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. |
| Background: | Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. FUNCTION: Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. TISSUE SPECIFICITY: Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Buffer: | Lyophilised in PBS |
| Application Summary: | immunohistochemistry, Western Blot 1:300 |
| Species Summary: | Hu |
| Alternate Names: | anti-Melanopsin antibody, anti-MGC142118 antibody, anti-MOP antibody, anti-OPN 4 antibody, anti-OPN4 antibody, anti-Opsin 4 antibody, anti-Opsin4 antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
| Notes Main: | Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| General References: | 1. Provencio I, et al. J. Neurosci. 20:600-605(2000). 2. Melyan Z, et al. Nature 433:741-745(2005). 3. Kumbalasiri T, et al. Photochem. Photobiol. 83 (2), 273-279 (2007). 4.Dacey D.M, et al. Nature 433 (7027), 749-754 (2005). 5. Qiu X, et al. Nature 433 (7027), 745-749 (2005). 6. Fu G.K, et al. Genomics 84 (1), 205-210 (2004). |
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