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| Catalog Code: | NB110-67828 |
| Product Name: | Beta-actin Antibody |
| Product Description: | Mouse Monoclonal anti-Beta-actin (AC-15) |
| Clone: | AC-15 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 2.0 mg/ml |
| Immunogen: | Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). |
| Epitope: | N-terminus of the beta isoform of actin. |
| Cross Reactivity: | Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp and goldfish), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat, and Sheep. Detects beta actin in a wide variety of tissues and species. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. |
| Packaging: | 0.1 ml Affinity purified Mouse ascites. |
| Uses: | Useful as an extract loading control for a wide variety of cell extracts in Western blot. The epitope recognized by the antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives). |
| Background: | This is an ideal reagent for use as a cell extract loading control on Western blot. It has been used as such in most of the references below. Molecular weight of beta actin is 42kD. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 0.01 M PBS, pH 7.4 |
| Application Summary: | ELISA , immunohistochemistry , immunofluorescence , Western Blot 0.5 - 1 ug/ml, Immunocytochemistry 10 - 40 ug/ml, Acetone Fixation |
| Species Summary: | Bv, Ca, Ch, Dr, Hu, Mu, Po, Rt, Rb, Gp, Fe, Fi, Sh, , , , , |
| Alternate Names: | Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actin antibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actin antibody, Anti-Beta actin antibody, Anti-Actin, beta antibody |
| Package Size: | 0.1 ml |
| Preservative: | 15mm sodium azide |
| Notes Main: | Immunofluoresence staining of chicken gizzard ultra-thin sections (North et al. J. Cell Sci. 107:445-455 (1994)) labels the dense bodies, longitudinal channels and membrane associated dense-plaque. |
| Product References: | 1. North, A.J., et al. J. Cell Sci. 107, 445-455, (1994) 2. Gimona, M., et al. Cell Motil., 108-116, (1994) 3. Gimona, M., et al., â-actin specific monoclonal antibody. Cell. Motil. Cytoskel. 27, 108-116, (1994) 4. North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994) 5. North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994) 6. Liao, J., et al., Direct reprobing with anti-â-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000) 7. Abramovich, D., Parborell, F., and Tesone, M. (2006) Effect of a Vascular Endothelial Growth Factor (VEGF) Inhibitory Treatment on the Folliculogenesis and Ovarian Apoptosis in Gonadotropin-Treated Prepubertal Rats, 75, 434-441. |
| Novus References: | 1. Hawkins, B., et al. Inhibition of Insulin Synthesis by Cypoheptadine: Effects on Translation. Toxicological Sciences. 79:258-265, 2004. 2. Thornton, M., et al. Degradation of NF-KB in T Cells by Gangliosides Expressed on Renal Cell Carcinomas. The Journal of Immunology. 172: 3480-3490, 2004. (Western blot, human) 3. Chong, V., et al. Differential display polymerase chain reaction reveals increased expression of striatal rat glia-derived nexin following chronic clozapine treatment. The Pharmacogenomics Journal. 4: 379-387, 2004. 4. Sung, S., et al. Modulation of Nuclear Factor-kappaB Activity by Indomethacin Influences ABeta Levels but Not ABeta Precursor Protein Metabolism in a Model of Alzheimer's Disease. Am. J. Pathol. 165: 2197-2206, 2004. 5. Sun, D., et al. Reduced release of Nitric Oxide to Shear Stress in Mesenteric Arteries of Aged Rats. Am J Physiol Heart Circ Physiol. 286: 2249-2256, 2004. 6. Adler, S., et al. Oxidant Stress in Kidneys of Spontaneously Hypertensive Rats Involves Both Oxidase Overexpression and Loss of Extracellular Superoxide Dismutase. Am J Physiol Renal Physiol. 287:907-913, 2004. 7. Adler, S., et al. Oxidant Stress Leads to Impaired Regulation of Renal Cortical Oxygen Consumption by Nitric Oxide in the Aging Kidney. Journal of the American Society of Nephrology. 15: 52-60, 2004. 8. Mukherjee, P., Antiangiogenic and Proapoptotic Effects of Dietary Restriction on Experimental Mouse and Human Brain Tumors. Clinic Cancer Research. 10: 5622-5629, 2004. 9. Mukherjee, P., et al. Antiangiogenic and Proapoptotic Effects of Dietary Restriction on Experimental Mouse and Human Brain Tumors. Clinical Cancer Research. 10: 5622-5629, 2004. 10. Laughlin, R., Involvement of Raft-Like Plasma Membrane Domains of Entamoeba Histolytica in Pinocytosis and Adhesion. Infection and Immunity. 72: 5349-5357, 2004. 11. Watters, J., et al. Genome-wide Discovery of Loci Influencing Chemotherapy Cytotoxicity. PNAS. 101: 11809-11814, 2004. 12. Dietz, A., et al. Imatinib mesylate inhibits T-cell proliferation in vitro and delayed-type hypersensitivity in vivo. Blood. 104: 1094-1099, 2004. 13. Rice, P.L., et al. Sulindac independently modulates extracellular signal-regulated kinase 1/2 and cyclic GMP-dependent protein kinase signaling pathways. Mol. Cancer Ther. 5:746-754, 2006. 14. Liu, X., & Manser, T. Antibuclear antigen B cells that down-regulate surface B cell receptor during development to mature follicular pheotype do not display features of anergy in vitro. J. Immunol. 174:4505-4515, 2005. 15. Moiseyev, G., et al. RPE65 is the isomerohydrolase in the retinoid visual cycle. PNAS. 102:12413-12418, 2005. 16. Csiszar, A., et al. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotypes. Am. J. Pathol. 168:629-638, 2006. 17. Dennis, Vida A.., Host Response and Inflammation: Interleukin-10 Anti-Inflammatory Response to Borrelia burgdorferi, the Agent of Lyme Diseassse: a Possible role for Suppressors of Cytokine Signaling 1 and 3. 74: 5780-5789, 2006. 18. Abramovich, Dalhia., et al. Ovary: effect of a vascular endothelial growth factor (VEGF) Ingibitory treatment on th efolliculogenesis and ovarian apoptosis in gonadotropin-treated prepuberal rats. 75: 434-441, 2006. 19. Greer, E.L. et al. MECHANISMS OF SIGNAL TRANSDUCTION: The Energy Sensor AMP-activated Protein Kinase Directly Regulates the Mammalian FOXO3 Transcription Factor. J. Biol. Chem. 282:30107-30119, Oct 2007. 20. Park, K., et al. Conditional Neuronal Simian Virus 40 T Antigen Expression Induces Alzheimer-Like Tau and Amyloid Pathology in Mice. J.Neurosci., Mar 2007; 27: 2969 -2978. 21. Ougolkov, A., et al. Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor B target genes and induction of apoptosis in chronic lymphocytic leukemia B cells. Blood, Jul 2007; 110: 735 - 742. 22. Zhang, S., et al. OSU-03012, a Novel Celecoxib Derivative, Is Cytotoxic to Myeloma Cells and Acts through Multiple Mechanisms. Clin. Cancer Res. 2007 13: 4750-4758. 23. Alejandro EU, Johnson JD. Inhibition of Raf-1 Alters Multiple Downstream Pathways to Induce Pancreatic -Cell Apoptosis. J Biol Chem. 2008 January 25, 2008;283(4):2407-17. |
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