| CatalogCode: | NB110-66720 |
| ProductName: | PKC zeta Antibody |
| Product Description: | Rabbit Monoclonal anti-PKC zeta (EP2037AY) [Thr560] |
| Clone: | EP2037AY |
| Clonality: | Monoclonal |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Threonine 560 of human PKC zeta was used as an immunogen. |
| Specificity: | This antibody detects PKC zeta phosphorylated at Threonine 560. |
| CrossReactivity: | This antibody is reactive against Human, Mouse, and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunocytochemistry. |
| Control: | HeLa cell lysate, Human brain and Human Breast Tissue. |
| Background: | Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 295 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | Anti-nPKC zeta antibody, Anti-OTTHUMP00000044160 antibody, Anti-PKC 2 antibody, Anti-PKC2 antibody, Anti-PRKCZ antibody, Anti-Protein kinase C zeta antibody, Anti-Protein kinase C zeta type antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Kochs G, et al. Eur J Biochem 216(2):597-606, 1993 2. Chou MM, et al. Curr Biol 8(19):1069-77 |
| Phosphorylated: | Thr560 |
|