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| Catalog Code: | NB110-66666 |
| Product Name: | JNK3 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-JNK3 (EP1597Y) [Tyr223] |
| Clone: | EP1597Y |
| Clonality: | Monoclonal |
| ListPrice: | 320 |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of human JNK3 was used as immunogen. |
| Specificity: | The antibody will detect JNK3 phosphorylation on tyrosine 223. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot and Immunoprecipitation. |
| Background: | C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3). |
| Storage: | Aliquot and store at -20 °C. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation 1:20, Western Blot 1:5000-1:20000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-Stress-activated protein kinase JNK3 antibody, Anti-c-Jun N-terminal kinase 3 antibody, Anti-MAP kinase p49 3F12 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr223 |
| General References: | 1. Kuan CY, et al. PNAS 100(25):15184-89, 2003 2. Fleming Y, et al. Biochem J 352(Pt1):145-54, 2000 3. Gupta S, et al. EMBO J. 15:2760-2770, 1996. |
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