| CatalogCode: | NB110-66665 |
| ProductName: | MSK1 Antibody |
| Product Description: | Rabbit Monoclonal anti-MSK1 (EP1888Y) [Ser360] |
| Clone: | EP1888Y |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding Serine 360 of human MSK1 was used as immunogen. |
| Specificity: | The antibody will detect MSK1 phosphorylation on Serine 360. |
| CrossReactivity: | This antibody is reactive against Human, Mouse, and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot, Immunocytochemistry, and Immunoprecipitation. |
| Control: | HEK293 cell lysate |
| Background: | MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 295 |
| AppSummary: | IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | Anti-90 kDa Ribosomal Protein S6 Kinase 5 antibody, Anti-MSPK1 antibody, Anti-Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody, Anti-Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody, Anti-Ribosomal Protein S6 Kinase Alpha 5 antibody, Anti-RLPK antibody, Anti-RPS6KA5 antibody, Anti-RSK Like Protein Kinase antibody, Anti-RSKL antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Deak M, Clifton AD, Lucocq LM, Alessi DR, EMBO J 1998;17:4426-41 2. Pierrat et al. (1998) J.Biol. Chem. 273, 29661 3. New et al (1999) J. Biol. Chem. 274, 1026-18852 4. Claire E. McCoy et al. Biochemical Journal Immediate Publication.November 29, 2004 |
| Phosphorylated: | Ser360 |
|