| CatalogCode: | NB110-66661 |
| ProductName: | MARCKS Antibody |
| Product Description: | Rabbit Monoclonal anti-MARCKS (EP2113Y) [Ser159/Ser163] |
| Clone: | EP2113Y |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. |
| Specificity: | This antibody detects the residues surrounding serine 159 and 163 of human MARCKS protein. |
| CrossReactivity: | This antibody is reactive agains Human, Mouse, and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot and Immunoprecipitation. |
| Control: | HeLa cell lysate |
| Background: | The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phospholyation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 295 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | Anti-80K L protein antibody, Anti-80KL antibody, Anti-81 kDa protein, light chain antibody, Anti-MACS antibody, Anti-MARCS antibody, Anti-MGC52672 antibody, Anti-Myristoylated alanine rich protein kinase C substrate antibody, Anti-Phosphomyristin antibody, Anti-PKCSL antibody, Anti-PRKCSL antibody, Anti-Protein kinase C substrate antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Harlan DM, et al. J Biol Chem 266(22):14399-405, 1991 2. Hartwig JH, et al. Nature 356(6370):618-22, 1992. |
| Phosphorylated: | Ser159/Ser163 |
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